PKM1 Exerts Critical Roles in Cardiac Remodeling Under Pressure ...
WEBJun 9, 2021 · PKM1 transgenic mice were generated under the control of tetracycline response elements, and cardiac-specific overexpression of PKM1 was induced by doxycycline administration in adult mice. Pressure overload was triggered by transverse aortic constriction.
PKM1 Confers Metabolic Advantages and Promotes Cell ... - Cell …
WEBHere we show that expression of PKM1, rather than PKM2, activates glucose metabolism and promotes tumor growth cell autonomously in a KrasLSL-G12D mouse model and in KRAS G12V MEFs. We propose that activation of autophagy/mitophagy confers a metabolic advantage mediated by PKM1, favoring malignancy.
PKM1 Confers Metabolic Advantages and Promotes Cell
WEBMar 12, 2018 · Here we show that expression of PKM1, rather than PKM2, activates glucose metabolism and promotes tumor growth cell autonomously in a KrasLSL-G12D mouse model and in KRAS G12V MEFs. We propose that activation of autophagy/mitophagy confers a metabolic advantage mediated by …
Loss of pyruvate kinase M2 limits growth and triggers innate …
WEBOct 9, 2018 · Loss of endothelial PKM2 alters mitochondrial metabolism. The PKM gene is abundantly expressed in ECs (Supplementary Figure 1a), and RT-qPCR (Fig. 1a) and western blot (Fig. 1b) analyses show...
β-cell deletion of the PKm1 and PKm2 isoforms of pyruvate kinase …
WEBAug 23, 2022 · This manuscript employs in vitro studies and elegant mouse models to detail how specific pyruvate kinase isoforms impact pancreatic β-cell ATP/ADP levels, ATP-sensitive K + channel (KATP channel) activity, calcium handling, and insulin secretion.
WEBABSTRACT. Isoform selection of pyruvate kinase M (PKM), a glycolytic enzyme, influences fates of glucose-derived carbons in cellular metabolic networks. We recently developed novel mouse lines to study PKM isoform function and identified PKM1 as a potential target in a subset of human lung cancers.
Pyruvate Kinase M2 Protects Heart from Pressure …
WEBJun 3, 2022 · The results showed that cardiomyocyte‐specific Pkm2 deletion resulted in significant deterioration of cardiac functions under pressure overload, whereas Pkm2 overexpression mitigated transverse aortic constriction‐induced cardiac hypertrophy and improved heart functions.
Modulation of PKM activity affects the differentiation of T
WEBImmunoblot analysis confirmed PKM1 protein expression in PKM2-deficient cells, which was not detectable in CD4 + T cells from WT mice (fig. S1B). These data demonstrate that deletion of PKM2 may be functionally compensated by an increase in PKM1 expression.
Role of pyruvate kinase M2-mediated metabolic reprogramming …
WEBMay 11, 2020 · Meanwhile, podocyte-specific Pkm2-knockout (KO) mice developed worse albuminuria and podocyte injury after adriamycin treatment. We identified mammalian target of rapamycin (mTOR) as a...
Enzymatic activation of pyruvate kinase increases cytosolic ...
WEBJul 5, 2021 · PKM2 knockout induces PKM1 expression in some tumour cells in mice; however, the PKM1-expressing tumour cells were less proliferative than the tumour cells with complete knockout of both PKM1...
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